LEO Pharma Open Innovation

T-cell inflammation assay

Human CD4+ T-cells are presented with beads coated in CD2, CD3 & CD28, to induce an inflammatory response, detected as an increase in IL-2 & IL-4 secretion. Your compound is tested for its ability to inhibit this inflammatory response, measured as an inhibition of IL-2 & IL-4 release. Cellular proliferation is also assessed, as a measure of general compound cytotoxicity.