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Testing Molecules

LEO Pharma Open Innovation offers free testing of undisclosed molecules in several advanced bioassays relevant for inflammation. This enables the discovery of novel chemistry of both early and late-stage drug molecules as well as drug target exploration and validation using tool molecules.

Our disease-relevant inflammatory bioassay toolbox currently include (but maybe be subject to change):

Keratinocyte CCL2 release assay

Primary human keratinocytes are stimulated with IL-4, IL-13, IL-22 and IFN-gamma into a state of eczema-relevant inflammation resulting in an increased secretion of the CCL2 cytokine. We test your molecule for its ability to inhibit the inflammation by reducing CCL2 secretion.

More about the CCL2 keratinocyte assay.

T-cell inflammation assay

Primary human CD4+ T-cells are presented with beads coated with CD2, CD3 & CD28 antibodies to induce an string inflammatory response, which is detected as an increase in IL-2 & IL-4 secretion. We test your molecule for its ability to inhibit this inflammatory response.

More about the IL-2 and IL-4 T-cell assay.

PBMC IL-17 assay

Primary human peripheral blood mononuclear cells (PBMCs) are presented with beads coated in CD3 & CD28 and exposed to IL-23 in the medium to induce an inflammatory response, which is detected as an increase in IL-17 secretion. We test your molecule for its ability to inhibit this inflammatory response.

More about the IL-17 PBMC release assay.

New Assay to come

Additional assays are being developed and will be online later this year.

Assay not online.

The critical contractual points in the open innovation agreement

We believe in transparency, trust and mutual benefits to optimize collaborations. Our agreement includes:

  The structure of your molecules remains secret
  No business obligations - you are in full control all throughout the process
  Disclosing the science  - you know how the molecules are tested
  You own all the data that we generate while testing your molecules
  This service is free of charge

Download the LEO Pharma Open Innovation agreement here

The process explained in 4 steps:                                                

1. Signing of the user agreement.
2. Ship your molecules in the provided vials.
3. We test your molecules in our laboratories.
4. Receive a report with all the data and results.

Here is an example report of what the data could look like.

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