Keratinocyte IL-8 release assay
Primary human keratinocytes (skin cells) are stimulated with a combination of TNF-alpha and IL-17 to induce a psoriasis-relevant response, detected as an increase in IL-8 release. Your compound is tested for the ability to inhibit the inflammatory response, measured as an inhibition of IL-8 levels. Cell viability is also assessed, as a measure of general compound cytotoxicity.
Download the assay protocol.
The Keratinocyte IL-8 release assay procedure
The assay utilizes primary human keratinocytes seeded in a 384-well microtiter plate and stimulated with the two cytokines IL-17 and TNF-alpha in order to induced secretion of the proinflammatory cytokine IL-8. The test molecule, dissolved in DMSO, is added in different concentrations and after 72 hours the IL-8 levels, as well as the cell viability is measured as the final end point.
