The Keratinocyte IL-8 release assay procedure
The assay utilizes primary human keratinocytes seeded in a 384-well microtiter plate and stimulated with the two cytokines IL-17 and TNF-alpha in order to induced secretion of the proinflammatory cytokine IL-8. The test molecule, dissolved in DMSO, is added in different concentrations and after 72 hours the IL-8 levels, as well as the cell viability is measured as the final end point.